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rat pulmonary artery smooth muscle cells pasmcs  (Dawley Inc)

 
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    Dawley Inc rat pulmonary artery smooth muscle cells pasmcs
    Rat Pulmonary Artery Smooth Muscle Cells Pasmcs, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pasmcs/cells+muscle+primary+smooth+vascular/pm41780607-68-0-13
    Average 86 stars, based on 1 article reviews
    rat pulmonary artery smooth muscle cells pasmcs - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    Isolation:

    Article Title: NCAM1 modulates the proliferation and migration of pulmonary arterial smooth muscle cells in pulmonary hypertension.
    Article Snippet: .. Primary PASMCs were isolated from Sprague–Dawley rats (6–8 weeks, 180–200 g) as previously described [22]. ..

    Article Title: Exosomes derived from mesenchymal stromal cells exert a therapeutic effect on hypoxia-induced pulmonary hypertension by modulating the YAP1/SPP1 signaling pathway.
    Article Snippet: .. PASMCs were extracted from the pulmonary artery of Sprague Dawley (SD) rats weighing 80–100 g. The specific extraction technique may vary but typically involves the isolation and dissociation of smooth muscle cells from the pulmonary artery tissue. ..

    Article Title: NCAM1 modulates the proliferation and migration of pulmonary arterial smooth muscle cells in pulmonary hypertension
    Article Snippet: .. Primary PASMCs were isolated from Sprague–Dawley rats (6–8 weeks, 180–200 g) as previously described [ ]. ..

    Article Title: TNFα Induces DNA and Histone Hypomethylation and Pulmonary Artery Smooth Muscle Cell Proliferation Partly via Excessive Superoxide Formation
    Article Snippet: .. Methods: PASMCs were isolated from 8-week-old male Sprague-Dawley rats and treated with 0, 20, or 200 ng/mL TNF-α for 24 or 48 h. After treatment, cell number, superoxide production, histone acetylation, DNA methylation, and histone methylation were assessed. ..

    Article Title: Identification of potential biomarkers associated with mitochondrial oxidative stress in idiopathic pulmonary arterial hypertension via bioinformatic and experimental analysis
    Article Snippet: The lung tissue cells were subsequently counterstained with DAPI (Servicebio) and slices were observed under a microscope. .. The isolation of primary PASMCs was performed from main pulmonary arteries of male Sprague-Dawley rats weighing 100–150 g. The rats were first anesthetized with an intraperitoneal injection of 50 mg/kg sodium pentobarbital and subsequently sterilized by immersion in 75% alcohol for 3 min. ..

    Article Title: HMGB1-induced activation of ER stress contributes to pulmonary artery hypertension in vitro and in vivo.
    Article Snippet: .. Primary PASMCs were isolated and cultured from pulmonary arteries of male Sprague–Dawley rats (120– 180 g) as previously described [28]. ..

    Extraction:

    Article Title: Exosomes derived from mesenchymal stromal cells exert a therapeutic effect on hypoxia-induced pulmonary hypertension by modulating the YAP1/SPP1 signaling pathway.
    Article Snippet: .. PASMCs were extracted from the pulmonary artery of Sprague Dawley (SD) rats weighing 80–100 g. The specific extraction technique may vary but typically involves the isolation and dissociation of smooth muscle cells from the pulmonary artery tissue. ..

    Ex Vivo:

    Article Title: The Xanthine Derivative KMUP-1 Inhibits Hypoxia-Induced TRPC1 Expression and Store-Operated Ca 2+ Entry in Pulmonary Arterial Smooth Muscle Cells.
    Article Snippet: .. Ex vivo PASMCs were cultured from Sprague-Dawley rats in a modular incubator chamber under 1% O2/5% CO2 for 24 h to elucidate TRPC1 overexpression and observe the Ca2+ release and entry. ..

    Cell Culture:

    Article Title: The Xanthine Derivative KMUP-1 Inhibits Hypoxia-Induced TRPC1 Expression and Store-Operated Ca 2+ Entry in Pulmonary Arterial Smooth Muscle Cells.
    Article Snippet: .. Ex vivo PASMCs were cultured from Sprague-Dawley rats in a modular incubator chamber under 1% O2/5% CO2 for 24 h to elucidate TRPC1 overexpression and observe the Ca2+ release and entry. ..

    Article Title: HMGB1-induced activation of ER stress contributes to pulmonary artery hypertension in vitro and in vivo.
    Article Snippet: .. Primary PASMCs were isolated and cultured from pulmonary arteries of male Sprague–Dawley rats (120– 180 g) as previously described [28]. ..

    Incubator:

    Article Title: The Xanthine Derivative KMUP-1 Inhibits Hypoxia-Induced TRPC1 Expression and Store-Operated Ca 2+ Entry in Pulmonary Arterial Smooth Muscle Cells.
    Article Snippet: .. Ex vivo PASMCs were cultured from Sprague-Dawley rats in a modular incubator chamber under 1% O2/5% CO2 for 24 h to elucidate TRPC1 overexpression and observe the Ca2+ release and entry. ..

    Over Expression:

    Article Title: The Xanthine Derivative KMUP-1 Inhibits Hypoxia-Induced TRPC1 Expression and Store-Operated Ca 2+ Entry in Pulmonary Arterial Smooth Muscle Cells.
    Article Snippet: .. Ex vivo PASMCs were cultured from Sprague-Dawley rats in a modular incubator chamber under 1% O2/5% CO2 for 24 h to elucidate TRPC1 overexpression and observe the Ca2+ release and entry. ..

    DNA Methylation Assay:

    Article Title: TNFα Induces DNA and Histone Hypomethylation and Pulmonary Artery Smooth Muscle Cell Proliferation Partly via Excessive Superoxide Formation
    Article Snippet: .. Methods: PASMCs were isolated from 8-week-old male Sprague-Dawley rats and treated with 0, 20, or 200 ng/mL TNF-α for 24 or 48 h. After treatment, cell number, superoxide production, histone acetylation, DNA methylation, and histone methylation were assessed. ..

    Methylation:

    Article Title: TNFα Induces DNA and Histone Hypomethylation and Pulmonary Artery Smooth Muscle Cell Proliferation Partly via Excessive Superoxide Formation
    Article Snippet: .. Methods: PASMCs were isolated from 8-week-old male Sprague-Dawley rats and treated with 0, 20, or 200 ng/mL TNF-α for 24 or 48 h. After treatment, cell number, superoxide production, histone acetylation, DNA methylation, and histone methylation were assessed. ..

    Injection:

    Article Title: Identification of potential biomarkers associated with mitochondrial oxidative stress in idiopathic pulmonary arterial hypertension via bioinformatic and experimental analysis
    Article Snippet: The lung tissue cells were subsequently counterstained with DAPI (Servicebio) and slices were observed under a microscope. .. The isolation of primary PASMCs was performed from main pulmonary arteries of male Sprague-Dawley rats weighing 100–150 g. The rats were first anesthetized with an intraperitoneal injection of 50 mg/kg sodium pentobarbital and subsequently sterilized by immersion in 75% alcohol for 3 min. ..



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    Image Search Results


    Effect of mitoTEMPO treatment on HIF‐1α stabilization in vitro. HIF‐1α protein levels were assessed by western blot, and steady‐state mRNA levels for lactate dehydrogenase A ( Ldha ) and pyruvate dehydrogenase kinase 1 ( Pdk1 ) by quantitative real‐time PCR in (a) CMT167 cells, (b) hPASMCs, and (c) mPASMCs, exposed to normoxia (21% O 2 ), severe hypoxia (1% O 2 ), or mild hypoxia (10% O 2 ) for 24 h and treated with triphenylphosphonium (TPP + ) (blue dots) or mitoTEMPO (MT) (red dots). Immunoblots shown are representative of three independent experiments. CMT167: mouse lung carcinoma epithelial cells; hPASMCs: human pulmonary artery smooth muscle cells; mPASMCs: mouse pulmonary artery smooth muscle cells. Densitometric analysis of HIF‐1α bands normalized to β‐Actin. Data are presented as mean ± SD. Statistical comparisons were made using two‐way ANOVA with Tukey's post hoc test ( n = 3 per group). (ns: no signal). Quantitative real‐time PCR data reflect mean ΔCt ± SD ( n = 3 per experimental group).

    Journal: Physiological Reports

    Article Title: The effect of mitoTEMPO on the development of hypoxia‐induced pulmonary hypertension in male mice

    doi: 10.14814/phy2.70804

    Figure Lengend Snippet: Effect of mitoTEMPO treatment on HIF‐1α stabilization in vitro. HIF‐1α protein levels were assessed by western blot, and steady‐state mRNA levels for lactate dehydrogenase A ( Ldha ) and pyruvate dehydrogenase kinase 1 ( Pdk1 ) by quantitative real‐time PCR in (a) CMT167 cells, (b) hPASMCs, and (c) mPASMCs, exposed to normoxia (21% O 2 ), severe hypoxia (1% O 2 ), or mild hypoxia (10% O 2 ) for 24 h and treated with triphenylphosphonium (TPP + ) (blue dots) or mitoTEMPO (MT) (red dots). Immunoblots shown are representative of three independent experiments. CMT167: mouse lung carcinoma epithelial cells; hPASMCs: human pulmonary artery smooth muscle cells; mPASMCs: mouse pulmonary artery smooth muscle cells. Densitometric analysis of HIF‐1α bands normalized to β‐Actin. Data are presented as mean ± SD. Statistical comparisons were made using two‐way ANOVA with Tukey's post hoc test ( n = 3 per group). (ns: no signal). Quantitative real‐time PCR data reflect mean ΔCt ± SD ( n = 3 per experimental group).

    Article Snippet: Mouse lung carcinoma epithelial (CMT167) cells (10032302, Merck, Germany) and human PASMCs (hPASMCs) (C‐12521, PromoCell, Germany) were purchased.

    Techniques: In Vitro, Western Blot, Real-time Polymerase Chain Reaction